Gene Prediction with Augustus

augustus —introns=on —cds=on —codingseq=on —species=tribolium Ensamble20K/GENOMABROCA.fasta.masked > GENOMABROCA.fasta.masked.augustus

opt/scripts/extractProteineAugustus.pl GENOMABROCA.fasta.masked.augustus > GENOMABROCA.fasta.masked.augustus.pep

Blast Annotation

time blast -p blastp -i GENOMABROCA.fasta.masked.augustus.pep.fasta -d ~/DBs/nr -e 1e-5 -o GENOMABROCA.fasta.masked.augustus.pep.fastaVsNr.bp.xml -m 7 -v 20 -b 20 -a 16

real 4046m57.964s
user 55112m2.194s
sys 484m28.357s