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View Mapping ESTs to Assemblies

Cenicafe Bauveria bassiana ESTs

grep -c ‘>’ BbassianaR50Cenicafe.fasta
7317

Mapping Cenicafe ESTs to Assemblies

/opt/blat/blat -t=dna Bb9001_trim_CLC.fasta -q=rna BbassianaR50Cenicafe.fasta -ooc=/opt/blat/11.ooc outputBb9001CLC.psl

Countig mapped ESTs sequences to Genome sequences

less outputBb9001CLC.psl | awk ‘{print $10}’ | sort | uniq | wc -l
7011

Assembly Bb9001 CLC Bb9001 Velvet Bb9024 CLC Bb9024 Velvet Bb9119 CLC Bb9119 Velvet Bb9205 CLC Bb9205 Velvet
#Hits 7011 6735 6981 6809 6918 6576 6937 6721

ESTs Not Hit with Assemblies

Cenicafe ESTs not in Bb9001 Assembly: 309
Cenicafe ESTs not in Bb9024 Assembly: 339
Cenicafe ESTs not in Bb9119 Assembly: 402
Cenicafe ESTs not in Bb9205 Assembly: 383

Diferences

ESTsBbassiana Not In Bb9001CLC Vs ESTs Bbassiana Not In Bb9024CLC : 66
ESTsBbassiana Not In Bb9001CLC Vs ESTs Bbassiana Not In Bb9119CLC : 113
ESTsBbassiana Not In Bb9001CLC Vs ESTs Bbassiana Not In Bb9205CLC : 102

ESTsBbassiana Not In Bb9024CLC Vs ESTs Bbassiana Not In Bb9001CLC : 66
ESTsBbassiana Not In Bb9024CLC Vs ESTs Bbassiana Not In Bb9119CLC: 87
ESTsBbassiana Not In Bb9024CLC Vs ESTs Bbassiana Not In Bb9205CLC: 70

ESTsBbassiana Not In Bb9119CLC Vs ESTs Bbassiana Not In Bb9205CLC: 43
ESTsBbassiana Not In Bb9119CLC Vs ESTs Bbassiana Not In Bb9024CLC: 87
ESTsBbassiana Not In Bb9119CLC Vs ESTs Bbassiana Not In Bb9001CLC: 113

diff -y idsESTsBbassianaNotInBb9119CLC idsESTsBbassianaNotInBb9205CLC | grep -e ‘>’ -e ‘<’ | wc -l
43

File:tablaESTsNotInBbAssembly.xlsx